MOBKL1A ELISA Kit

Catalog No. ABIN6951675

Product Details MOBKL1A ELISA Kit

Target: MOBKL1A (MOB1B) (MOB Kinase Activator 1B (MOB1B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human MOB1B ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for MOBKL1A

Target: MOBKL1A (MOB1B) (MOB Kinase Activator 1B (MOB1B))

Alternative Name: MOB1B (MOB1B Products)

Synonyms: MATS2 ELISA Kit, MOB4A ELISA Kit, MOBKL1A ELISA Kit, 1110003E08Rik ELISA Kit, AU015450 ELISA Kit, B230364F10 ELISA Kit, Mobkl1a ELISA Kit, mobkl1a ELISA Kit, mobkl1aa ELISA Kit, wu:fj65h03 ELISA Kit, zgc:56156 ELISA Kit, zgc:77872 ELISA Kit, MOB1B ELISA Kit, mob4a ELISA Kit, mats2 ELISA Kit, C2orf6 ELISA Kit, MOB1 ELISA Kit, MOBK1B ELISA Kit, Mob4B ELISA Kit, cb512 ELISA Kit, mobkl1ab ELISA Kit, sb:cb512 ELISA Kit, zgc:111840 ELISA Kit, MOB kinase activator 1B ELISA Kit, MOB kinase activator 1Ba ELISA Kit, Mps one binder kinase activator-like 1A ELISA Kit, mps one binder kinase activator-like 1A ELISA Kit, MOB kinase activator 1B L homeolog ELISA Kit, MOB kinase activator 1Bb ELISA Kit, MOB1B ELISA Kit, Mob1b ELISA Kit, mob1ba ELISA Kit, mol1a ELISA Kit, LOC100282811 ELISA Kit, LOC100285953 ELISA Kit, mob1b ELISA Kit, mob1b.L ELISA Kit, mob1bb ELISA Kit

Gene ID: 92597

UniProt: Q7L9L4