DCUN1D2 ELISA Kit
DCUN1D2
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Target
See all DCUN1D2 products
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DCUN1D2
(DCN1, Defective in Cullin Neddylation 1, Domain Containing 2 (DCUN1D2))
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Reactivity
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Human
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Detection Method
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Colorimetric
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Method Type
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Sandwich ELISA
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Application
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ELISA
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Purpose
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Human DCUN1D2 ELISA Kit.
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Sample Type
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Application Notes
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Optimal working dilution should be determined by the investigator.
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Plate
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Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Expiry Date
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6 months
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Target
See all DCUN1D2 products
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DCUN1D2
(DCN1, Defective in Cullin Neddylation 1, Domain Containing 2 (DCUN1D2))
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Alternative Name
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DCUN1D2 (DCUN1D2 Products)
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Synonyms
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C13orf17 ELISA Kit, RGD1311743 ELISA Kit, AW495713 ELISA Kit, mFLJ10704 ELISA Kit, defective in cullin neddylation 1 domain containing 2 ELISA Kit, DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae) ELISA Kit, DCUN1D2 ELISA Kit, Dcun1d2 ELISA Kit
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Gene ID
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55208
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UniProt
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Q6PH85
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