EPH Receptor A4 ELISA Kit

Catalog No. ABIN6951637

Product Details EPH Receptor A4 ELISA Kit

Target: EPH Receptor A4 (EPHA4)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human EphA4 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for EPH Receptor A4

Target: EPH Receptor A4 (EPHA4)

Alternative Name: EphA4 (EPHA4 Products)

Synonyms: HEK8 ELISA Kit, SEK ELISA Kit, TYRO1 ELISA Kit, RGD1560587 ELISA Kit, ephe ELISA Kit, EPHA4 ELISA Kit, Ci-Eph1 ELISA Kit, sek ELISA Kit, hek8 ELISA Kit, rtk1 ELISA Kit, tyr01 ELISA Kit, 2900005C20Rik ELISA Kit, AI385584 ELISA Kit, Cek8 ELISA Kit, Hek8 ELISA Kit, Sek ELISA Kit, Sek1 ELISA Kit, Tyro1 ELISA Kit, rb ELISA Kit, XEpha4 ELISA Kit, sek-1 ELISA Kit, epha4 ELISA Kit, pag ELISA Kit, EphA4 ELISA Kit, apha3 ELISA Kit, ek2 ELISA Kit, epha3 ELISA Kit, sb:cb246 ELISA Kit, zek2 ELISA Kit, zgc:109882 ELISA Kit, EPH receptor A4 ELISA Kit, Eph receptor A4 ELISA Kit, ephrin receptor epsilon ELISA Kit, EPH receptor A4 S homeolog ELISA Kit, EPH receptor A4 L homeolog ELISA Kit, eph receptor A4a ELISA Kit, EPHA4 ELISA Kit, Epha4 ELISA Kit, ephe ELISA Kit, epha4 ELISA Kit, epha4.S ELISA Kit, epha4.L ELISA Kit, epha4a ELISA Kit

Gene ID: 2043

UniProt: P54764

Pathways: RTK Signaling