CES5A ELISA Kit

Catalog No. ABIN6951576

Product Details CES5A ELISA Kit

Target: CES5A (Carboxylesterase 5A (CES5A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse CES5A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for CES5A

Target: CES5A (Carboxylesterase 5A (CES5A))

Alternative Name: CES5A (CES5A Products)

Synonyms: CAUXIN ELISA Kit, CES4C1 ELISA Kit, CES5 ELISA Kit, CES7 ELISA Kit, 1700081L16Rik ELISA Kit, 1700122C07Rik ELISA Kit, BB081581 ELISA Kit, Ces7 ELISA Kit, Gm503 ELISA Kit, cauxin ELISA Kit, Ces5 ELISA Kit, LOC445455 ELISA Kit, carboxylesterase 5A ELISA Kit, CES5A ELISA Kit, Ces5a ELISA Kit

Gene ID: 67935

UniProt: Q6AW46