HAAO ELISA Kit

Catalog No. ABIN6951566

Product Details HAAO ELISA Kit

Target: HAAO (3-hydroxyanthranilate 3,4-Dioxygenase (HAAO))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Rat HAAO ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for HAAO

Target: HAAO (3-hydroxyanthranilate 3,4-Dioxygenase (HAAO))

Alternative Name: HAAO (HAAO Products)

Synonyms: Afu2g17450 ELISA Kit, 0610007K21Rik ELISA Kit, 0610012J07Rik ELISA Kit, 3-HAO ELISA Kit, 3-HAOxase ELISA Kit, 3HAO ELISA Kit, zgc:103585 ELISA Kit, HAO ELISA Kit, 3-hydroxyanthranilate 3,4-dioxygenase ELISA Kit, 3-hydroxyanthranilate 3,4-dioxygenase L homeolog ELISA Kit, SPO1774 ELISA Kit, CNB05620 ELISA Kit, AFUA_2G17450 ELISA Kit, Bcep18194_C7641 ELISA Kit, Kfla_5491 ELISA Kit, Micau_4935 ELISA Kit, RBRH_03005 ELISA Kit, Krodi_1278 ELISA Kit, haao ELISA Kit, HAAO ELISA Kit, Haao ELISA Kit, haao.L ELISA Kit

Gene ID: 56823

UniProt: P46953