Beclin 1 ELISA Kit

Catalog No. ABIN6951538

Product Details Beclin 1 ELISA Kit

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Binding Specificity: pSer234

Reactivity: Human, Rat, Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human/Mouse/Rat Phospho-Beclin-1 (S234) ELISA Kit. This assay semi-quantitatively measures phosphorylated Beclin-1 (Ser234) in lysate samples.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes human, mouse, and rat Beclin-1 phosphorylated at site Serine-234.

Characteristics:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: Beclin-1 (BECN1 Products)

Synonyms: beclin-1 ELISA Kit, atg6 ELISA Kit, vps30 ELISA Kit, beclin1 ELISA Kit, Beclin-1 ELISA Kit, becn1 ELISA Kit, zgc:73097 ELISA Kit, ATG6 ELISA Kit, VPS30 ELISA Kit, 4921513J16Rik ELISA Kit, 5430417M23Rik ELISA Kit, Atg6 ELISA Kit, beclin 1 ELISA Kit, beclin 1, autophagy related ELISA Kit, beclin 1 protein ELISA Kit, beclin-1 ELISA Kit, beclin family protein ELISA Kit, beclin 1, autophagy related L homeolog ELISA Kit, BECN1 ELISA Kit, becn1 ELISA Kit, atg6 ELISA Kit, CpipJ_CPIJ011087 ELISA Kit, PTRG_07825 ELISA Kit, SJAG_01876 ELISA Kit, Becn1 ELISA Kit, becn1.L ELISA Kit

Gene ID: 8678

Pathways: Autophagy