ATG4A ELISA Kit

Catalog No. ABIN6951537

Product Details ATG4A ELISA Kit

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ATG4A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for ATG4A

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Alternative Name: ATG4A (ATG4A Products)

Synonyms: apg4a ELISA Kit, autl2 ELISA Kit, F6E13.27 ELISA Kit, APG4A ELISA Kit, zgc:111958 ELISA Kit, Aut2a ELISA Kit, AUTL2 ELISA Kit, AI627006 ELISA Kit, AV169859 ELISA Kit, Apg4a ELISA Kit, Atg4al ELISA Kit, Autl2 ELISA Kit, Atg4a ELISA Kit, autophagy related 4A cysteine peptidase L homeolog ELISA Kit, autophagy related 4A cysteine peptidase ELISA Kit, Peptidase family C54 protein ELISA Kit, ATG4 autophagy related 4 homolog A (S. cerevisiae) ELISA Kit, autophagy related 4A, cysteine peptidase ELISA Kit, atg4a.L ELISA Kit, ATG4A ELISA Kit, AT2G44140 ELISA Kit, atg4a ELISA Kit, Atg4a ELISA Kit

Gene ID: 115201

UniProt: Q8WYN0

Pathways: Autophagy