ATG5 ELISA Kit

Catalog No. ABIN6951530

Product Details ATG5 ELISA Kit

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ATG5 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5 (ATG5 Products)

Synonyms: APG5 ELISA Kit, APG5-LIKE ELISA Kit, APG5L ELISA Kit, ASP ELISA Kit, hAPG5 ELISA Kit, ATG5 ELISA Kit, CG1643 ELISA Kit, DmAtg5 ELISA Kit, Dmel\\CG1643 ELISA Kit, atg5 ELISA Kit, 2010107M05Rik ELISA Kit, 3110067M24Rik ELISA Kit, AW319544 ELISA Kit, Apg5l ELISA Kit, Atg5l ELISA Kit, C88337 ELISA Kit, Paddy ELISA Kit, apg5l ELISA Kit, zgc:100934 ELISA Kit, ATATG5 ELISA Kit, AUTOPHAGY 5 ELISA Kit, MKP11.20 ELISA Kit, MKP11_20 ELISA Kit, autophagy related 5 ELISA Kit, Autophagy-related 5 ELISA Kit, ATG5 autophagy related 5 homolog (S. cerevisiae) ELISA Kit, autophagy related 5 L homeolog ELISA Kit, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 ELISA Kit, autophagy protein Apg5 family ELISA Kit, ATG5 ELISA Kit, Atg5 ELISA Kit, atg5 ELISA Kit, atg5.L ELISA Kit, APG5 ELISA Kit

Gene ID: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy