ATG4B ELISA Kit

Catalog No. ABIN6951529

Product Details ATG4B ELISA Kit

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ATG4B ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Alternative Name: ATG4B (ATG4B Products)

Synonyms: apg4b ELISA Kit, APG4B ELISA Kit, AUTL1 ELISA Kit, 2510009N07Rik ELISA Kit, AW048066 ELISA Kit, Apg4b ELISA Kit, Atg4bl ELISA Kit, Autl1 ELISA Kit, Aut2b2 ELISA Kit, autophagy related 4B cysteine peptidase ELISA Kit, autophagy-related 4b ELISA Kit, Peptidase family C54 protein ELISA Kit, cysteine protease ATG4B ELISA Kit, ATG4 autophagy related 4 homolog B (S. cerevisiae) ELISA Kit, autophagy related 4B, cysteine peptidase ELISA Kit, autophagy related 4B, cysteine peptidase L homeolog ELISA Kit, ATG4B ELISA Kit, Atg4b ELISA Kit, AT3G59950 ELISA Kit, Tsp_08582 ELISA Kit, Tsp_11599 ELISA Kit, atg4b ELISA Kit, atg4b.L ELISA Kit

Gene ID: 23192

UniProt: Q9Y4P1

Pathways: Autophagy