ATG16L1 ELISA Kit

Catalog No. ABIN6951527

Product Details ATG16L1 ELISA Kit

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ATG16L1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for ATG16L1

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Alternative Name: ATG16L1 (ATG16L1 Products)

Synonyms: APG16L ELISA Kit, ATG16A ELISA Kit, ATG16L ELISA Kit, IBD10 ELISA Kit, WDR30 ELISA Kit, 1500009K01Rik ELISA Kit, Apg16l ELISA Kit, Atg16l ELISA Kit, Wdr30 ELISA Kit, atg16 ELISA Kit, atg16l ELISA Kit, fb57e05 ELISA Kit, wu:fb57e05 ELISA Kit, zgc:110147 ELISA Kit, autophagy related 16 like 1 ELISA Kit, autophagy related 16-like 1 (S. cerevisiae) ELISA Kit, autophagy related 16-like 1 ELISA Kit, autophagy related 16 like 1 S homeolog ELISA Kit, ATG16 autophagy related 16-like 1 (S. cerevisiae) ELISA Kit, ATG16L1 ELISA Kit, Atg16l1 ELISA Kit, atg16l1 ELISA Kit, atg16l1.S ELISA Kit

Gene ID: 55054

UniProt: Q676U5

Pathways: Autophagy