14-3-3 theta ELISA Kit

Catalog No. ABIN6951519

Product Details 14-3-3 theta ELISA Kit

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human 14-3-3 theta ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3 theta (YWHAQ Products)

Synonyms: 14-3-3 ELISA Kit, 14-3-3t ELISA Kit, 1c5 ELISA Kit, hs1 ELISA Kit, 1C5 ELISA Kit, HS1 ELISA Kit, 2700028P07Rik ELISA Kit, AA409740 ELISA Kit, AU021156 ELISA Kit, R74690 ELISA Kit, ywhaq ELISA Kit, ywhaq2 ELISA Kit, wu:fa91b01 ELISA Kit, wu:fb02e12 ELISA Kit, wu:fb36f06 ELISA Kit, wu:fk86h02 ELISA Kit, ywhaq1 ELISA Kit, ywhaz ELISA Kit, zgc:55499 ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta S homeolog ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide a ELISA Kit, 14-3-3 protein theta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide b ELISA Kit, YWHAQ ELISA Kit, ywhaq ELISA Kit, Ywhaq ELISA Kit, ywhaq.S ELISA Kit, ywhaqa ELISA Kit, ywhaqb ELISA Kit

Gene ID: 10971

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction