CD8 alpha ELISA Kit

Catalog No. ABIN6951505

Product Details CD8 alpha ELISA Kit

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Hispid Cotton Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Sigmodon CD8A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8A (CD8A Products)

Synonyms: cd8 ELISA Kit, LOC100136450 ELISA Kit, BB154331 ELISA Kit, Ly-2 ELISA Kit, Ly-35 ELISA Kit, Ly-B ELISA Kit, Lyt-2 ELISA Kit, zgc:136643 ELISA Kit, CD8 ELISA Kit, Leu2 ELISA Kit, MAL ELISA Kit, p32 ELISA Kit, RHACD8-4 ELISA Kit, CD8a molecule ELISA Kit, uncharacterized LOC100125537 ELISA Kit, CD8 alpha ELISA Kit, CD8 antigen, alpha chain ELISA Kit, T-cell surface glycoprotein CD8 alpha chain ELISA Kit, Cd8a ELISA Kit, LOC100125537 ELISA Kit, LOC100136450 ELISA Kit, CD8A ELISA Kit, cd8a ELISA Kit, LOC100126556 ELISA Kit

Gene ID: 24930

UniProt: P07725

Pathways: TCR Signaling