m1ip1 ELISA Kit

Catalog No. ABIN6951500

Product Details m1ip1 ELISA Kit

Target: m1ip1 (Mid1-Interacting Protein 1 (m1ip1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human MID1IP1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for m1ip1

Target: m1ip1 (Mid1-Interacting Protein 1 (m1ip1))

Alternative Name: MID1IP1 (m1ip1 Products)

Synonyms: MIG12 ELISA Kit, TB10 ELISA Kit, G12-like ELISA Kit, S14R ELISA Kit, STRAIT11499 ELISA Kit, THRSPL ELISA Kit, 3110038L01Rik ELISA Kit, Mig12 ELISA Kit, slap ELISA Kit, mig12 ELISA Kit, thrspl ELISA Kit, MGC80850 ELISA Kit, zgc:73223 ELISA Kit, g12 ELISA Kit, wu:fj32f05 ELISA Kit, thymosin beta 10 ELISA Kit, MID1 interacting protein 1 ELISA Kit, Mid1-interacting protein 1 ELISA Kit, Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) ELISA Kit, MID1 interacting protein 1 S homeolog ELISA Kit, MID1 interacting protein 1b ELISA Kit, MID1 interacting protein 1a ELISA Kit, TMSB10 ELISA Kit, MID1IP1 ELISA Kit, m1ip1 ELISA Kit, Mid1ip1 ELISA Kit, mid1ip1.S ELISA Kit, mid1ip1b ELISA Kit, mid1ip1a ELISA Kit

Gene ID: 58526

UniProt: Q9NPA3