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UBE2D1 ELISA Kit

UBE2D1 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6951479
  • Target See all UBE2D1 products
    UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human UBE2D1 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Expiry Date
    6 months
  • Target See all UBE2D1 products
    UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))
    Alternative Name
    UBE2D1 (UBE2D1 Products)
    Synonyms
    E2(17)KB1 ELISA Kit, SFT ELISA Kit, UBC4/5 ELISA Kit, UBCH5 ELISA Kit, UBCH5A ELISA Kit, ube2d1 ELISA Kit, wu:fc16h06 ELISA Kit, zgc:73096 ELISA Kit, e2(17)kb1 ELISA Kit, sft ELISA Kit, ubc4/5 ELISA Kit, ubch5 ELISA Kit, ubch5a ELISA Kit, ubiquitin conjugating enzyme E2 D1 ELISA Kit, ubiquitin-conjugating enzyme E2D 1 ELISA Kit, ubiquitin-conjugating enzyme E2D 1b ELISA Kit, ubiquitin conjugating enzyme E2 D1 S homeolog ELISA Kit, UBE2D1 ELISA Kit, Ube2d1 ELISA Kit, ube2d1b ELISA Kit, ube2d1.S ELISA Kit, ube2d1 ELISA Kit
    Gene ID
    7321
    UniProt
    P51668
    Pathways
    Activation of Innate immune Response, Toll-Like Receptors Cascades
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