IRAK4 ELISA Kit

Catalog No. ABIN6951467

Product Details IRAK4 ELISA Kit

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human IRAK4 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Alternative Name: IRAK4 (IRAK4 Products)

Synonyms: zgc:55553 ELISA Kit, IRAK4 ELISA Kit, ipd1 ELISA Kit, irak-4 ELISA Kit, ny-ran-64 ELISA Kit, ny-ren-64 ELISA Kit, ren64 ELISA Kit, irak4 ELISA Kit, IPD1 ELISA Kit, IRAK-4 ELISA Kit, NY-REN-64 ELISA Kit, REN64 ELISA Kit, 8430405M07Rik ELISA Kit, 9330209D03Rik ELISA Kit, interleukin 1 receptor associated kinase 4 ELISA Kit, interleukin-1 receptor-associated kinase 4 ELISA Kit, IRAK4 ELISA Kit, irak4 ELISA Kit, Irak4 ELISA Kit

Gene ID: 51135

UniProt: Q9NWZ3

Pathways: NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades