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M-CSF/CSF1 ELISA Kit

CSF1 Reactivity: Mouse Colorimetric Sandwich ELISA 1 pg/mL - 350 pg/mL Cell Lysate, Tissue Lysate
Catalog No. ABIN6951452
  • Target See all M-CSF/CSF1 (CSF1) ELISA Kits
    M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
    Reactivity
    • 11
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    1 pg/mL - 350 pg/mL
    Minimum Detection Limit
    1 pg/mL
    Application
    ELISA
    Purpose
    Mouse M-CSF ELISA Kit for cell and tissue lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product CSF1 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all M-CSF/CSF1 (CSF1) ELISA Kits
    M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
    Alternative Name
    M-CSF (CSF1 Products)
    Synonyms
    CSF-1 ELISA Kit, MCSF ELISA Kit, C87615 ELISA Kit, Csfm ELISA Kit, op ELISA Kit, csf1-1 ELISA Kit, zgc:172186 ELISA Kit, CSF1 ELISA Kit, csf1-2 ELISA Kit, zgc:158436 ELISA Kit, colony stimulating factor 1 ELISA Kit, colony stimulating factor 1 (macrophage) ELISA Kit, colony stimulating factor 1a (macrophage) ELISA Kit, macrophage colony-stimulating factor 1 ELISA Kit, macrophage colony stimulating factor ELISA Kit, colony stimulating factor 1b (macrophage) ELISA Kit, CSF1 ELISA Kit, Csf1 ELISA Kit, csf1a ELISA Kit, LOC396599 ELISA Kit, LOC100860895 ELISA Kit, csf1b ELISA Kit
    Gene ID
    12977
    UniProt
    P07141
    Pathways
    RTK Signaling
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