HRAS ELISA Kit

Catalog No. ABIN6951446

Product Details HRAS ELISA Kit

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human HRAS ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name: HRAS (HRAS Products)

Synonyms: C-BAS/HAS ELISA Kit, C-H-RAS ELISA Kit, C-HA-RAS1 ELISA Kit, CTLO ELISA Kit, H-RASIDX ELISA Kit, HAMSV ELISA Kit, HRAS1 ELISA Kit, K-RAS ELISA Kit, N-RAS ELISA Kit, RASH1 ELISA Kit, hras ELISA Kit, zgc:110250 ELISA Kit, HRAS ELISA Kit, H-RAS ELISA Kit, c-H-ras ELISA Kit, H-Ras ELISA Kit, K-Ras ELISA Kit, hras1 ELISA Kit, rash1 ELISA Kit, ras ELISA Kit, N-Ras ELISA Kit, c-bas/has ELISA Kit, H-ras ELISA Kit, Ha-ras ELISA Kit, Harvey-ras ELISA Kit, Hras-1 ELISA Kit, Kras2 ELISA Kit, c-Ha-ras ELISA Kit, c-rasHa ELISA Kit, hrasl ELISA Kit, zgc:110734 ELISA Kit, HRas proto-oncogene, GTPase ELISA Kit, v-Ha-ras Harvey rat sarcoma viral oncogene homolog a ELISA Kit, neuroblastoma RAS viral (v-ras) oncogene homolog pseudogene ELISA Kit, Harvey rat sarcoma viral oncogene homolog L homeolog ELISA Kit, Harvey rat sarcoma viral oncogene homolog ELISA Kit, Harvey rat sarcoma virus oncogene ELISA Kit, NRAS proto-oncogene, GTPase ELISA Kit, -Ha-ras Harvey rat sarcoma viral oncogene homolog b ELISA Kit, HRAS ELISA Kit, hrasa ELISA Kit, LOC733587 ELISA Kit, Hras ELISA Kit, hras.L ELISA Kit, hras ELISA Kit, NRAS ELISA Kit, hrasb ELISA Kit

Gene ID: 3265

UniProt: P01112

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling