DREAM ELISA Kit

Catalog No. ABIN6951435

Product Details DREAM ELISA Kit

Target: DREAM (KCNIP3) (Kv Channel Interacting Protein 3, Calsenilin (KCNIP3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human KCNIP3 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for DREAM

Target: DREAM (KCNIP3) (Kv Channel Interacting Protein 3, Calsenilin (KCNIP3))

Alternative Name: KCNIP3 (KCNIP3 Products)

Synonyms: CSEN ELISA Kit, DREAM ELISA Kit, KCHIP3 ELISA Kit, 4933407H12Rik ELISA Kit, AI413860 ELISA Kit, Csen ELISA Kit, KChIP3 ELISA Kit, R74849 ELISA Kit, Dream ELISA Kit, rKChIP3 ELISA Kit, csen ELISA Kit, im:7145308 ELISA Kit, kcnip3 ELISA Kit, zgc:73306 ELISA Kit, KCNIP3 ELISA Kit, potassium voltage-gated channel interacting protein 3 ELISA Kit, Kv channel-interacting protein 4 ELISA Kit, calsenilin ELISA Kit, Kv channel interacting protein 3, calsenilin ELISA Kit, Kv channel interacting protein 3b, calsenilin ELISA Kit, KCNIP3 ELISA Kit, LOC5568664 ELISA Kit, CpipJ_CPIJ002549 ELISA Kit, Tsp_05009 ELISA Kit, Kcnip3 ELISA Kit, kcnip3b ELISA Kit, kcnip3 ELISA Kit

Gene ID: 30818

UniProt: Q9Y2W7