TBCB ELISA Kit

Catalog No. ABIN6951431

Product Details TBCB ELISA Kit

Target: TBCB (Tubulin Folding Cofactor B (TBCB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human TBCB ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for TBCB

Target: TBCB (Tubulin Folding Cofactor B (TBCB))

Alternative Name: TBCB (TBCB Products)

Synonyms: CG22 ELISA Kit, CKAP1 ELISA Kit, CKAPI ELISA Kit, 2410007D12Rik ELISA Kit, AU041393 ELISA Kit, Ckap1 ELISA Kit, ZH14 ELISA Kit, ckap1 ELISA Kit, ik:tdsubc_2e4 ELISA Kit, wu:fa56d03 ELISA Kit, xx:tdsubc_2e4 ELISA Kit, zgc:55620 ELISA Kit, TBCB ELISA Kit, cg22 ELISA Kit, ckapi ELISA Kit, CG11242 ELISA Kit, Dmel\CG11242 ELISA Kit, dTBCB ELISA Kit, tubulin folding cofactor B ELISA Kit, tubulin folding cofactor B S homeolog ELISA Kit, tubulin-folding cofactor B ELISA Kit, tubulin-binding cofactor B ELISA Kit, TBCB ELISA Kit, Tbcb ELISA Kit, tbcb ELISA Kit, tbcb.S ELISA Kit, EMB2804 ELISA Kit, LOC9320850 ELISA Kit

Gene ID: 1155

UniProt: Q99426