CRIP2 ELISA Kit

Catalog No. ABIN6951411

Product Details CRIP2 ELISA Kit

Target: CRIP2 (Cysteine-Rich Protein 2 (CRIP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human CRIP2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for CRIP2

Target: CRIP2 (Cysteine-Rich Protein 2 (CRIP2))

Alternative Name: CRIP2 (CRIP2 Products)

Synonyms: CRIP ELISA Kit, CRP2 ELISA Kit, ESP1 ELISA Kit, C6orf210 ELISA Kit, COQ10D3 ELISA Kit, DLP1 ELISA Kit, bA59I9.3 ELISA Kit, hDLP1 ELISA Kit, AW551867 ELISA Kit, Crp2 ELISA Kit, SmLim ELISA Kit, SmLIM ELISA Kit, zgc:77740 ELISA Kit, 0610010I23Rik ELISA Kit, AW743261 ELISA Kit, C77570 ELISA Kit, CRP4 ELISA Kit, Crp ELISA Kit, Hlp ELISA Kit, wu:fb30h10 ELISA Kit, zgc:55408 ELISA Kit, cysteine rich protein 2 ELISA Kit, decaprenyl diphosphate synthase subunit 2 ELISA Kit, cysteine and glycine-rich protein 2 ELISA Kit, cysteine and glycine rich protein 2 ELISA Kit, cysteine-rich protein 2 ELISA Kit, CRIP2 ELISA Kit, PDSS2 ELISA Kit, Csrp2 ELISA Kit, csrp2 ELISA Kit, CSRP2 ELISA Kit, Crip2 ELISA Kit, crip2 ELISA Kit

Gene ID: 1397

UniProt: P52943