CAMK4 ELISA Kit
CAMK4
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Target
See all CAMK4 ELISA Kits
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CAMK4
(Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))
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Reactivity
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Human
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Detection Method
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Colorimetric
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Method Type
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Sandwich ELISA
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Application
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ELISA
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Purpose
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Human CAMK4 ELISA Kit.
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Sample Type
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Top Product
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Discover our top product CAMK4 ELISA Kit
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Application Notes
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Optimal working dilution should be determined by the investigator.
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Plate
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Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Expiry Date
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6 months
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Target
See all CAMK4 ELISA Kits
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CAMK4
(Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))
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Alternative Name
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CAMK4 (CAMK4 Products)
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Synonyms
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CaMK IV ELISA Kit, CaMK-GR ELISA Kit, IV ELISA Kit, caMK ELISA Kit, CAMK4 ELISA Kit, A430110E23Rik ELISA Kit, AI666733 ELISA Kit, CaMKIV ELISA Kit, CaMKIV/Gr ELISA Kit, D18Bwg0362e ELISA Kit, Ccdpk ELISA Kit, RATCCDPK ELISA Kit, camk4-A ELISA Kit, wu:fj42a03 ELISA Kit, zgc:110275 ELISA Kit, calcium/calmodulin dependent protein kinase IV ELISA Kit, calcium/calmodulin-dependent protein kinase IV ELISA Kit, calcium/calmodulin-dependent protein kinase IV L homeolog ELISA Kit, CAMK4 ELISA Kit, Camk4 ELISA Kit, camk4.L ELISA Kit, camk4 ELISA Kit
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Gene ID
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814
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UniProt
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Q16566
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Pathways
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EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Production of Molecular Mediator of Immune Response, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma
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