BNIP3L/NIX ELISA Kit

Catalog No. ABIN6951385

Product Details BNIP3L/NIX ELISA Kit

Target: BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human BNIP3L ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for BNIP3L/NIX

Target: BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))

Alternative Name: BNIP3L (BNIP3L Products)

Synonyms: BNIP3a ELISA Kit, NIX ELISA Kit, NIP3 ELISA Kit, Nix ELISA Kit, Nip3 ELISA Kit, C86132 ELISA Kit, D14Ertd719e ELISA Kit, Nip3L ELISA Kit, bnip3l ELISA Kit, nip3 ELISA Kit, zgc:110478 ELISA Kit, bnip3l2 ELISA Kit, wu:fb52b03 ELISA Kit, wu:fc02h04 ELISA Kit, BCL2 interacting protein 3 like ELISA Kit, BCL2 interacting protein 3 ELISA Kit, BCL2/adenovirus E1B interacting protein 3 ELISA Kit, BCL2/adenovirus E1B interacting protein 3-like ELISA Kit, BCL2/adenovirus E1B interacting protein 3-like b ELISA Kit, BCL2/adenovirus E1B interacting protein 3-like a ELISA Kit, BNIP3L ELISA Kit, BNIP3 ELISA Kit, Bnip3l ELISA Kit, Bnip3 ELISA Kit, bnip3lb ELISA Kit, bnip3la ELISA Kit

Gene ID: 665

UniProt: O60238

Pathways: Autophagy