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GABARAP ELISA Kit

GABARAP Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6951379
  • Target See all GABARAP ELISA Kits
    GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human GABARAP ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Expiry Date
    6 months
  • Target See all GABARAP ELISA Kits
    GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))
    Alternative Name
    GABARAP (GABARAP Products)
    Synonyms
    GABARAP ELISA Kit, ATG8A ELISA Kit, GABARAP-a ELISA Kit, MM46 ELISA Kit, gabarap ELISA Kit, mg:bb02b03 ELISA Kit, GABA type A receptor-associated protein ELISA Kit, gamma-aminobutyric acid receptor-associated protein ELISA Kit, GABA(A) receptor-associated protein a ELISA Kit, gamma-aminobutyric acid receptor associated protein ELISA Kit, GABARAP ELISA Kit, LOC5568843 ELISA Kit, Gabarap ELISA Kit, gabarapa ELISA Kit
    Gene ID
    11337
    UniProt
    O95166
    Pathways
    Autophagy
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