SNAP25 ELISA Kit

Catalog No. ABIN6951376

Product Details SNAP25 ELISA Kit

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human SNAP25 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP25 (SNAP25 Products)

Synonyms: RIC-4 ELISA Kit, RIC4 ELISA Kit, SEC9 ELISA Kit, SNAP ELISA Kit, SNAP-25 ELISA Kit, bA416N4.2 ELISA Kit, dJ1068F16.2 ELISA Kit, SNAP-25B ELISA Kit, SNAP-25a ELISA Kit, ric-4 ELISA Kit, ric4 ELISA Kit, sec9 ELISA Kit, snap-25 ELISA Kit, Bdr ELISA Kit, sp ELISA Kit, fj55h11 ELISA Kit, snap25 ELISA Kit, wu:fj55h11 ELISA Kit, Snap ELISA Kit, snap25.2 ELISA Kit, wu:fj33h05 ELISA Kit, synaptosome associated protein 25 ELISA Kit, synaptosome associated protein 25kDa S homeolog ELISA Kit, synaptosomal-associated protein, 25kDa ELISA Kit, synaptosomal-associated protein 25 ELISA Kit, synaptosome associated protein 25kDa ELISA Kit, synaptosome associated protein 25kDa L homeolog ELISA Kit, zgc:101731 ELISA Kit, synaptosomal-associated protein, 25b ELISA Kit, SNAP25 ELISA Kit, Snap25 ELISA Kit, snap25.S ELISA Kit, snap25 ELISA Kit, BACOIKO003_07 ELISA Kit, Tsp_10811 ELISA Kit, snap25.L ELISA Kit, zgc:101731 ELISA Kit, snap25b ELISA Kit

Gene ID: 6616

UniProt: P60880

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport