NT5C3 ELISA Kit

Catalog No. ABIN6951367

Product Details NT5C3 ELISA Kit

Target: NT5C3 (5'-Nucleotidase, Cytosolic III (NT5C3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human NT5C3A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for NT5C3

Target: NT5C3 (5'-Nucleotidase, Cytosolic III (NT5C3))

Alternative Name: NT5C3A (NT5C3 Products)

Synonyms: 1600024P05Rik ELISA Kit, 2610206B05Rik ELISA Kit, 3110004A18Rik ELISA Kit, AI842776 ELISA Kit, Nt5c3a ELISA Kit, Nt5y ELISA Kit, PN-1 ELISA Kit, PN-I ELISA Kit, PSN1 ELISA Kit, UMPH ELISA Kit, Umph-1 ELISA Kit, Umph1 ELISA Kit, cN-III ELISA Kit, lupin ELISA Kit, p36 ELISA Kit, fb30h07 ELISA Kit, nt5c3 ELISA Kit, wu:fb30h07 ELISA Kit, zgc:66117 ELISA Kit, NT5C3 ELISA Kit, PYN1 ELISA Kit, P5'N-1 ELISA Kit, P5N-1 ELISA Kit, POMP ELISA Kit, UMPH1 ELISA Kit, hUMP1 ELISA Kit, Nt5c3 ELISA Kit, 5'-nucleotidase, cytosolic III ELISA Kit, 5'-nucleotidase, cytosolic IIIA ELISA Kit, cytosolic 5'-nucleotidase 3A ELISA Kit, Nt5c3 ELISA Kit, nt5c3a ELISA Kit, NT5C3A ELISA Kit, LOC534181 ELISA Kit, Nt5c3a ELISA Kit, nt5c3a.L ELISA Kit, Bm1_56095 ELISA Kit

Gene ID: 51251

UniProt: Q9H0P0