PEBP1 ELISA Kit

Catalog No. ABIN6951355

Product Details PEBP1 ELISA Kit

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human PEBP1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for PEBP1

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Alternative Name: PEBP1 (PEBP1 Products)

Synonyms: HCNP ELISA Kit, HCNPpp ELISA Kit, PBP ELISA Kit, PEBP ELISA Kit, PEBP-1 ELISA Kit, RKIP ELISA Kit, pbp ELISA Kit, hcnp ELISA Kit, pebp ELISA Kit, rkip ELISA Kit, Pbp ELISA Kit, Pbp1 ELISA Kit, Pbpr ELISA Kit, Rkip ELISA Kit, PEBP1 ELISA Kit, BcDNA:LP12095 ELISA Kit, CG18594 ELISA Kit, Dmel\\CG18594 ELISA Kit, T2 ELISA Kit, zgc:56033 ELISA Kit, zgc:76942 ELISA Kit, phosphatidylethanolamine binding protein 1 ELISA Kit, phosphatidylethanolamine binding protein 1 L homeolog ELISA Kit, phosphatidylethanolamine-binding protein ELISA Kit, phosphatidylethanolamine-binding protein,putative ELISA Kit, Phosphatidylethanolamine-binding protein 1 ELISA Kit, PEBP1 ELISA Kit, pebp1 ELISA Kit, Pebp1 ELISA Kit, pebp1.L ELISA Kit, RR_RS07785 ELISA Kit, PVX_235290 ELISA Kit, PVX_123630 ELISA Kit, PKH_143640 ELISA Kit, EAM_1207 ELISA Kit

Gene ID: 5037

UniProt: P30086

Pathways: Feeding Behaviour