ATP5F1D ELISA Kit

Catalog No. ABIN6951353

Product Details ATP5F1D ELISA Kit

Target: ATP5F1D (ATP synthase subunit delta, mitochondrial (ATP5F1D))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ATP5D ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for ATP5F1D

Target: ATP5F1D (ATP synthase subunit delta, mitochondrial (ATP5F1D))

Alternative Name: ATP5D (ATP5F1D Products)

Synonyms: fk58f09 ELISA Kit, zgc:73303 ELISA Kit, wu:fk58f09 ELISA Kit, MGC85306 ELISA Kit, GB17255 ELISA Kit, DDBDRAFT_0216607 ELISA Kit, DDBDRAFT_0238330 ELISA Kit, DDB_0216607 ELISA Kit, DDB_0238330 ELISA Kit, 0610008F14Rik ELISA Kit, 1500000I11Rik ELISA Kit, AA960090 ELISA Kit, AI876556 ELISA Kit, AU020773 ELISA Kit, C85518 ELISA Kit, ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit ELISA Kit, ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit L homeolog ELISA Kit, ATP synthase F1 delta ELISA Kit, atp5d ELISA Kit, atp5d.L ELISA Kit, ATP5D ELISA Kit, LOC552682 ELISA Kit, atp5D ELISA Kit, Atp5d ELISA Kit

Gene ID: 513

UniProt: P30049

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process