AACS ELISA Kit

Catalog No. ABIN6951352

Product Details AACS ELISA Kit

Target: AACS (Acetoacetyl-CoA Synthetase (AACS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human AACS ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for AACS

Target: AACS (Acetoacetyl-CoA Synthetase (AACS))

Alternative Name: AACS (AACS Products)

Synonyms: sur-5 ELISA Kit, An08g04090 ELISA Kit, AO090001000376 ELISA Kit, ACSF1 ELISA Kit, SUR-5 ELISA Kit, 2210408B16Rik ELISA Kit, SUR5 ELISA Kit, zgc:56105 ELISA Kit, acetoacetyl-CoA synthetase ELISA Kit, bll4282 ELISA Kit, acetoacetyl-coa synthetase ELISA Kit, acetoacetate--CoA ligase ELISA Kit, acetoacetate-CoA ligase ELISA Kit, Acetoacetyl-CoA synthetase ELISA Kit, AACS ELISA Kit, LOC590598 ELISA Kit, aacs ELISA Kit, bll4282 ELISA Kit, Pfl01_3080 ELISA Kit, h16_A2860 ELISA Kit, Mflv_1473 ELISA Kit, ANI_1_594074 ELISA Kit, Pput_2653 ELISA Kit, VIBHAR_RS20435 ELISA Kit, AOR_1_648164 ELISA Kit, CC1G_04522 ELISA Kit, LACBIDRAFT_230952 ELISA Kit, PLES_33261 ELISA Kit, PA1997 ELISA Kit, OB0504 ELISA Kit, CBU_0766 ELISA Kit, acsA2 ELISA Kit, Sde_0006 ELISA Kit, Plav_1154 ELISA Kit, AZC_2243 ELISA Kit, SGR_RS30655 ELISA Kit, Aacs ELISA Kit, sur-5 ELISA Kit

Gene ID: 65985

UniProt: Q86V21

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis