NXPH3 ELISA Kit
NXPH3
Reactivity: Rat
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
Target
See all NXPH3 products
-
NXPH3
(Neurexophilin 3 (NXPH3))
-
Reactivity
-
Rat
-
Detection Method
-
Colorimetric
-
Method Type
-
Sandwich ELISA
-
Application
-
ELISA
-
Purpose
-
Rat Neurexophilin-3 ELISA Kit.
-
Sample Type
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytical Method
-
Quantitative
-
Characteristics
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Components
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Material not included
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
Application Notes
-
Optimal working dilution should be determined by the investigator.
-
Plate
-
Pre-coated
-
Protocol
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
Restrictions
-
For Research Use only
-
-
Expiry Date
-
6 months
-
-
Target
See all NXPH3 products
-
NXPH3
(Neurexophilin 3 (NXPH3))
-
Alternative Name
-
Neurexophilin-3 (NXPH3 Products)
-
Synonyms
-
NPH3 ELISA Kit, Nph3 ELISA Kit, neurexophilin 3 ELISA Kit, neurexophilin 3 S homeolog ELISA Kit, NXPH3 ELISA Kit, nxph3.S ELISA Kit, nxph3 ELISA Kit, Nxph3 ELISA Kit
-
UniProt
-
B2GVB5
-