Tetraspanin 7 ELISA Kit

Catalog No. ABIN6951346

Product Details Tetraspanin 7 ELISA Kit

Target: Tetraspanin 7 (TSPAN7)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse TSPAN7 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for Tetraspanin 7

Target: Tetraspanin 7 (TSPAN7)

Alternative Name: TSPAN7 (TSPAN7 Products)

Synonyms: A15 ELISA Kit, CCG-B7 ELISA Kit, CD231 ELISA Kit, DXS1692E ELISA Kit, MRX58 ELISA Kit, MXS1 ELISA Kit, TALLA-1 ELISA Kit, TM4SF2 ELISA Kit, TM4SF2b ELISA Kit, 1200014P11Rik ELISA Kit, AI323365 ELISA Kit, Cd231 ELISA Kit, Mxs1 ELISA Kit, PE31 ELISA Kit, R74651 ELISA Kit, TALLA ELISA Kit, Tm4sf2 ELISA Kit, tm4sf2 ELISA Kit, zgc:63823 ELISA Kit, wu:fa11g01 ELISA Kit, wu:fd18e11 ELISA Kit, wu:fl39g07 ELISA Kit, a15 ELISA Kit, ccg-b7 ELISA Kit, cd231 ELISA Kit, mrx58 ELISA Kit, mxs1 ELISA Kit, talla-1 ELISA Kit, tspan7 ELISA Kit, tetraspanin 7 ELISA Kit, tetraspanin 7 L homeolog ELISA Kit, TSPAN7 ELISA Kit, Tspan7 ELISA Kit, tspan7 ELISA Kit, tspan7.L ELISA Kit

Gene ID: 21912

UniProt: Q62283