SOD2 ELISA Kit

Catalog No. ABIN6951323

Product Details SOD2 ELISA Kit

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human SOD2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Alternative Name: SOD2 (SOD2 Products)

Synonyms: LOC100101896 ELISA Kit, MNSOD ELISA Kit, GB14346 ELISA Kit, sod2 ELISA Kit, MGC88869 ELISA Kit, Sod2 ELISA Kit, CG8905 ELISA Kit, Dmel\\CG8905 ELISA Kit, Mito SOD ELISA Kit, Mn SOD ELISA Kit, Mn-SOD ELISA Kit, Mn-SOD2 ELISA Kit, MnSOD ELISA Kit, MnSODII ELISA Kit, Mn[2+]SOD ELISA Kit, SOD ELISA Kit, SOD-2 ELISA Kit, SOD2 ELISA Kit, Sod-2 ELISA Kit, dSOD2 ELISA Kit, mitSOD2 ELISA Kit, mnSOD ELISA Kit, IPOB ELISA Kit, MVCD6 ELISA Kit, cb463 ELISA Kit, wu:fj33b01 ELISA Kit, zgc:73051 ELISA Kit, MnSOD ELISA Kit, manganese superoxide dismutase ELISA Kit, superoxide dismutase 2, mitochondrial ELISA Kit, superoxide dismutase 2 ELISA Kit, Mn superoxide dismutase ELISA Kit, Superoxide dismutase 2 (Mn) ELISA Kit, superoxide dismutase 2 L homeolog ELISA Kit, BDBG_07234 ELISA Kit, LOC100101896 ELISA Kit, MNSOD ELISA Kit, Sod2 ELISA Kit, sod2 ELISA Kit, LbMnSOD4 ELISA Kit, SOD2 ELISA Kit, LOC100282741 ELISA Kit, SOD-2 ELISA Kit, sod2.L ELISA Kit

Gene ID: 6648

UniProt: P04179

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling