ERCC1 ELISA Kit

Catalog No. ABIN6951286

Product Details ERCC1 ELISA Kit

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ERCC1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1 (ERCC1 Products)

Synonyms: COFS4 ELISA Kit, RAD10 ELISA Kit, UV20 ELISA Kit, Ercc-1 ELISA Kit, ercc1 ELISA Kit, T12H1.18 ELISA Kit, T12H1_18 ELISA Kit, UV REPAIR DEFICIENT 7 ELISA Kit, UVR7 ELISA Kit, ERCC excision repair 1, endonuclease non-catalytic subunit ELISA Kit, excision repair cross-complementing rodent repair deficiency, complementation group 1 ELISA Kit, DNA excision repair protein ERCC-1 ELISA Kit, nucleotide repair protein ELISA Kit, excision repair cross-complementation group 1 L homeolog ELISA Kit, excision repair cross-complementation group 1 ELISA Kit, ERCC1 ELISA Kit, Ercc1 ELISA Kit, CC1G_04546 ELISA Kit, CpipJ_CPIJ013903 ELISA Kit, PITG_13719 ELISA Kit, ercc1 ELISA Kit, ercc1.L ELISA Kit

Gene ID: 2067

UniProt: P07992

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response