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TNFAIP8 ELISA Kit

TNFAIP8 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6951264
  • Target See all TNFAIP8 products
    TNFAIP8 (Tumor Necrosis Factor, alpha-Induced Protein 8 (TNFAIP8))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human TNFAIP8 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Expiry Date
    6 months
  • Target See all TNFAIP8 products
    TNFAIP8 (Tumor Necrosis Factor, alpha-Induced Protein 8 (TNFAIP8))
    Alternative Name
    TNFAIP8 (TNFAIP8 Products)
    Synonyms
    MGC55331 ELISA Kit, zgc:55331 ELISA Kit, si:dkey-49m19.6 ELISA Kit, GG2-1 ELISA Kit, MDC-3.13 ELISA Kit, SCC-S2 ELISA Kit, SCCS2 ELISA Kit, AA987150 ELISA Kit, E130304C20Rik ELISA Kit, ENSMUSG00000073567 ELISA Kit, Gg2-1 ELISA Kit, Gm10539 ELISA Kit, Nded ELISA Kit, Ssc-2 ELISA Kit, tumor necrosis factor, alpha-induced protein 8-like 1 ELISA Kit, TNF alpha induced protein 8 S homeolog ELISA Kit, TNF alpha induced protein 8 ELISA Kit, tumor necrosis factor, alpha-induced protein 8 ELISA Kit, tnfaip8l1 ELISA Kit, tnfaip8.S ELISA Kit, TNFAIP8 ELISA Kit, Tnfaip8 ELISA Kit
    Gene ID
    25816
    UniProt
    O95379
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