TWIST1 ELISA Kit

Catalog No. ABIN6951241

Product Details TWIST1 ELISA Kit

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human TWIST1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for TWIST1

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Alternative Name: TWIST1 (TWIST1 Products)

Synonyms: scs ELISA Kit, Xtwi ELISA Kit, acs3 ELISA Kit, X-twi ELISA Kit, bpes2 ELISA Kit, bpes3 ELISA Kit, twist ELISA Kit, Xtwist ELISA Kit, MGC69439 ELISA Kit, twist1 ELISA Kit, ACS3 ELISA Kit, BPES2 ELISA Kit, BPES3 ELISA Kit, CRS1 ELISA Kit, SCS ELISA Kit, TWIST ELISA Kit, bHLHa38 ELISA Kit, AA960487 ELISA Kit, M-Twist ELISA Kit, Pde ELISA Kit, Ska10 ELISA Kit, Ska ELISA Kit, Twist ELISA Kit, pdt ELISA Kit, cTwist ELISA Kit, Twist1b ELISA Kit, twist-1 ELISA Kit, wu:fd04c01 ELISA Kit, zgc:101115 ELISA Kit, twist family bHLH transcription factor 1 ELISA Kit, twist family bHLH transcription factor 1 S homeolog ELISA Kit, twist basic helix-loop-helix transcription factor 1 ELISA Kit, twist homolog 1 (Drosophila) ELISA Kit, twist family bHLH transcription factor 1a ELISA Kit, TWIST1 ELISA Kit, twist1 ELISA Kit, twist1.S ELISA Kit, Twist1 ELISA Kit, twist1a ELISA Kit

Gene ID: 7291

UniProt: Q15672

Pathways: p53 Signaling, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity