ROM1 ELISA Kit

Catalog No. ABIN6951227

Product Details ROM1 ELISA Kit

Target: ROM1 (Retinal Outer Segment Membrane Protein 1 (ROM1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ROSP1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for ROM1

Target: ROM1 (Retinal Outer Segment Membrane Protein 1 (ROM1))

Alternative Name: ROSP1 (ROM1 Products)

Synonyms: ROM ELISA Kit, ROSP1 ELISA Kit, RP7 ELISA Kit, TSPAN23 ELISA Kit, ROM1 ELISA Kit, M101156 ELISA Kit, Rgsc1156 ELISA Kit, Rom-1 ELISA Kit, Rosp1 ELISA Kit, Tspan23 ELISA Kit, rds35 ELISA Kit, rom ELISA Kit, rosp1 ELISA Kit, tspan23 ELISA Kit, Rds ELISA Kit, zgc:56548 ELISA Kit, zgc:73336 ELISA Kit, zgc:77401 ELISA Kit, retinal outer segment membrane protein 1 ELISA Kit, rod outer segment membrane protein 1 ELISA Kit, retinal outer segment membrane protein 1 L homeolog ELISA Kit, retinal outer segment membrane protein 1b ELISA Kit, retinal outer segment membrane protein 1a ELISA Kit, ROM1 ELISA Kit, Rom1 ELISA Kit, rom1.L ELISA Kit, rom1b ELISA Kit, rom1a ELISA Kit

Gene ID: 6094

UniProt: Q03395