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Prosaposin ELISA Kit

PSAP Reactivity: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6951184
  • Target See all Prosaposin (PSAP) ELISA Kits
    Prosaposin (PSAP)
    Reactivity
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Rat PSAP ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product PSAP ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Expiry Date
    6 months
  • Target See all Prosaposin (PSAP) ELISA Kits
    Prosaposin (PSAP)
    Alternative Name
    PSAP (PSAP Products)
    Synonyms
    GLBA ELISA Kit, SAP1 ELISA Kit, AI037048 ELISA Kit, SGP-1 ELISA Kit, SGP1A ELISA Kit, cb759 ELISA Kit, wu:fa14a06 ELISA Kit, wu:fb36e02 ELISA Kit, wu:fb58g07 ELISA Kit, PSAP ELISA Kit, PSP-A ELISA Kit, SFTPA1B ELISA Kit, SP-A ELISA Kit, prosaposin ELISA Kit, surfactant protein A1 ELISA Kit, PSAP ELISA Kit, Psap ELISA Kit, psap ELISA Kit, LOC100011417 ELISA Kit, LOC100304726 ELISA Kit, LOC100526719 ELISA Kit, SFTPA1 ELISA Kit
    Gene ID
    25524
    UniProt
    P10960
    Pathways
    Positive Regulation of Endopeptidase Activity
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