STXBP1 ELISA Kit

Catalog No. ABIN6951178

Product Details STXBP1 ELISA Kit

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human STXBP1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for STXBP1

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Alternative Name: STXBP1 (STXBP1 Products)

Synonyms: MUNC18-1 ELISA Kit, NSEC1 ELISA Kit, P67 ELISA Kit, RBSEC1 ELISA Kit, UNC18 ELISA Kit, AI317162 ELISA Kit, AI326233 ELISA Kit, MMS10-G ELISA Kit, Ms10g ELISA Kit, Munc-18a ELISA Kit, Munc18-1 ELISA Kit, N-sec1 ELISA Kit, Rb-sec1 ELISA Kit, Sxtbp1 ELISA Kit, Unc18-1 ELISA Kit, Unc18h ELISA Kit, nsec1 ELISA Kit, fj43h10 ELISA Kit, stxbp1 ELISA Kit, wu:fj36d12 ELISA Kit, wu:fj43h10 ELISA Kit, zgc:114171 ELISA Kit, rop ELISA Kit, unc18 ELISA Kit, hunc18 ELISA Kit, rbsec1 ELISA Kit, munc18-1 ELISA Kit, MGC146482 ELISA Kit, ANC18HA ELISA Kit, NSEC1A ELISA Kit, Sec1 ELISA Kit, n-sec1 ELISA Kit, nSec1 ELISA Kit, rbSec1 ELISA Kit, rbSec1A ELISA Kit, rbSec1B ELISA Kit, UNC18A ELISA Kit, si:rp71-10d23.3 ELISA Kit, syntaxin binding protein 1 ELISA Kit, syntaxin binding protein 1a ELISA Kit, syntaxin binding protein 1 L homeolog ELISA Kit, syntaxin binding protein 1b ELISA Kit, STXBP1 ELISA Kit, Stxbp1 ELISA Kit, stxbp1a ELISA Kit, stxbp1 ELISA Kit, Tb09.160.0780 ELISA Kit, stxbp1.L ELISA Kit, stxbp1b ELISA Kit

Gene ID: 6812

UniProt: P61764

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport