MDM4-binding Protein ELISA Kit

Catalog No. ABIN6951175

Product Details MDM4-binding Protein ELISA Kit

Target: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human MDM4 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for MDM4-binding Protein

Target: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Alternative Name: MDM4 (MDM4 Products)

Synonyms: mdmx ELISA Kit, HDMX ELISA Kit, MDMX ELISA Kit, MRP1 ELISA Kit, wu:fa09h09 ELISA Kit, wu:fi33d10 ELISA Kit, 4933417N07Rik ELISA Kit, AA414968 ELISA Kit, AL023055 ELISA Kit, AU018793 ELISA Kit, AU021806 ELISA Kit, C85810 ELISA Kit, Mdmx ELISA Kit, MDM4, p53 regulator S homeolog ELISA Kit, MDM4, p53 regulator ELISA Kit, transformed mouse 3T3 cell double minute 4 ELISA Kit, mdm4.S ELISA Kit, MDM4 ELISA Kit, Mdm4 ELISA Kit, mdm4 ELISA Kit

Gene ID: 4194

UniProt: O15151

Pathways: Cell Division Cycle