MAX ELISA Kit

Catalog No. ABIN6951174

Product Details MAX ELISA Kit

Target: MAX (MYC Associated Factor X (MAX))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human MAX ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for MAX

Target: MAX (MYC Associated Factor X (MAX))

Alternative Name: MAX (MAX Products)

Synonyms: CG9648 ELISA Kit, Dmel\\CG9648 ELISA Kit, MAX ELISA Kit, bHLHd9 ELISA Kit, dMax ELISA Kit, dmax ELISA Kit, max ELISA Kit, bhlhd4 ELISA Kit, bhlhd5 ELISA Kit, bhlhd6 ELISA Kit, bhlhd7 ELISA Kit, bhlhd8 ELISA Kit, xmax2 ELISA Kit, xmax4 ELISA Kit, xmax ELISA Kit, AA960152 ELISA Kit, AI875693 ELISA Kit, bHLHd4 ELISA Kit, bHLHd5 ELISA Kit, bHLHd6 ELISA Kit, bHLHd7 ELISA Kit, bHLHd8 ELISA Kit, fa92b04 ELISA Kit, fb52a04 ELISA Kit, fb95h11 ELISA Kit, wu:fa92b04 ELISA Kit, wu:fb52a04 ELISA Kit, wu:fb95h11 ELISA Kit, zmax ELISA Kit, CG9648 gene product from transcript CG9648-RD ELISA Kit, MYC associated factor X L homeolog ELISA Kit, MYC associated factor X ELISA Kit, Max protein ELISA Kit, MYC associated factor X S homeolog ELISA Kit, myc associated factor X ELISA Kit, Max ELISA Kit, max.L ELISA Kit, MAX ELISA Kit, max ELISA Kit, max.S ELISA Kit

Gene ID: 4149

UniProt: P61244

Pathways: Mitotic G1-G1/S Phases