GCSH ELISA Kit

Catalog No. ABIN6951138

Product Details GCSH ELISA Kit

Target: GCSH (Glycine Cleavage System H Protein (GCSH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human GCSH ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for GCSH

Target: GCSH (Glycine Cleavage System H Protein (GCSH))

Alternative Name: GCSH (GCSH Products)

Synonyms: GCE ELISA Kit, NKH ELISA Kit, GCSH ELISA Kit, H-PROTEIN ELISA Kit, 1100001L02Rik ELISA Kit, 5730591C18Rik ELISA Kit, wu:fb14d10 ELISA Kit, wu:fw84b08 ELISA Kit, zgc:112535 ELISA Kit, BA5228 ELISA Kit, gce ELISA Kit, gcsh ELISA Kit, nkh ELISA Kit, wu:fd56h06 ELISA Kit, zgc:110557 ELISA Kit, zgc:92732 ELISA Kit, glycine cleavage system protein H ELISA Kit, glycine cleavage system protein H (aminomethyl carrier) ELISA Kit, glycine cleavage system lipoyl carrier protein GcvH ELISA Kit, glycine cleavage system H protein ELISA Kit, glycine cleavage system protein H (aminomethyl carrier), a ELISA Kit, glycine cleavage system carrier of aminomethyl moiety GcvH ELISA Kit, leucine rich repeat containing 34 ELISA Kit, glycine cleavage system protein H (aminomethyl carrier) L homeolog ELISA Kit, glycine cleavage system protein H (aminomethyl carrier), b ELISA Kit, GCSH ELISA Kit, Gcsh ELISA Kit, gcvH-2 ELISA Kit, gcvH ELISA Kit, gcsha ELISA Kit, LRRC34 ELISA Kit, gcsh.L ELISA Kit, gcshb ELISA Kit

Gene ID: 2653

UniProt: P23434