PRDM2 ELISA Kit

Catalog No. ABIN6951135

Product Details PRDM2 ELISA Kit

Target: PRDM2 (PR Domain Containing 2, with ZNF Domain (PRDM2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human PRDM2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for PRDM2

Target: PRDM2 (PR Domain Containing 2, with ZNF Domain (PRDM2))

Alternative Name: PRDM2 (PRDM2 Products)

Synonyms: MGC114763 ELISA Kit, prdm2 ELISA Kit, riz ELISA Kit, kmt8 ELISA Kit, riz1 ELISA Kit, riz2 ELISA Kit, mtb-zf ELISA Kit, humhoxy1 ELISA Kit, PRDM2 ELISA Kit, HUMHOXY1 ELISA Kit, KMT8 ELISA Kit, MTB-ZF ELISA Kit, RIZ ELISA Kit, RIZ1 ELISA Kit, RIZ2 ELISA Kit, 4833427P12Rik ELISA Kit, E330024L24Rik ELISA Kit, Gm1033 ELISA Kit, Gm19732 ELISA Kit, Riz ELISA Kit, Riz1 ELISA Kit, Znfpr1c1 ELISA Kit, PR domain containing 2, with ZNF domain S homeolog ELISA Kit, PR domain containing 2, with ZNF domain b ELISA Kit, PR domain containing 2, with ZNF domain ELISA Kit, PR domain zinc finger protein 2 ELISA Kit, PR/SET domain 2 ELISA Kit, prdm2.S ELISA Kit, prdm2b ELISA Kit, prdm2 ELISA Kit, LOC100350859 ELISA Kit, PRDM2 ELISA Kit, Prdm2 ELISA Kit

Gene ID: 7799

UniProt: Q13029