GCAP1 ELISA Kit

Catalog No. ABIN6951132

Product Details GCAP1 ELISA Kit

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human GUCA1A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for GCAP1

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Alternative Name: GUCA1A (GUCA1A Products)

Synonyms: C6orf131 ELISA Kit, COD3 ELISA Kit, CORD14 ELISA Kit, GCAP ELISA Kit, GCAP1 ELISA Kit, GUCA ELISA Kit, GUCA1 ELISA Kit, dJ139D8.6 ELISA Kit, MGC84170 ELISA Kit, GUCA1A ELISA Kit, guca1a ELISA Kit, MGC146317 ELISA Kit, GC-A ELISA Kit, Gcap1 ELISA Kit, Guca1 ELISA Kit, mGCAP1 ELISA Kit, gcap1 ELISA Kit, guanylate cyclase activator 1A ELISA Kit, guanylate cyclase activator 1A L homeolog ELISA Kit, guanylate cyclase activator 1A (retina) ELISA Kit, guanylate cyclase activator 1a (retina) ELISA Kit, GUCA1A ELISA Kit, Guca1a ELISA Kit, guca1a.L ELISA Kit, guca1a ELISA Kit

Gene ID: 2978

UniProt: P43080

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction