FKBP1A ELISA Kit

Catalog No. ABIN6951130

Product Details FKBP1A ELISA Kit

Target: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human FKBP12 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for FKBP1A

Target: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Alternative Name: FKBP12 (FKBP1A Products)

Synonyms: 12kDa ELISA Kit, FKBP12 ELISA Kit, FKBP12-T1 ELISA Kit, FKBP12-T2 ELISA Kit, Fkbp ELISA Kit, Fkbp1 ELISA Kit, mFKBP1 ELISA Kit, mFKBP12 ELISA Kit, FKBP-12 ELISA Kit, FKBP-1A ELISA Kit, FKBP1 ELISA Kit, Rotamase ELISA Kit, Fkbp12 ELISA Kit, ARABIDOPSIS THALIANA FK506-BINDING PROTEIN 12 ELISA Kit, ATFKBP12 ELISA Kit, FK506-binding protein 12 ELISA Kit, IMMUNOPHILIN ELISA Kit, MSJ1.19 ELISA Kit, MSJ1_19 ELISA Kit, PKC12 ELISA Kit, PKCI2 ELISA Kit, PPIASE ELISA Kit, FKBP1B ELISA Kit, Fkbp2 ELISA Kit, fj02g10 ELISA Kit, zgc:73373 ELISA Kit, wu:fj02g10 ELISA Kit, FKBP1A ELISA Kit, Bmfkbp13 ELISA Kit, zgc:103752 ELISA Kit, FK506 binding protein 1a ELISA Kit, FK506 binding protein 1A ELISA Kit, FK506-binding protein ELISA Kit, FK506-binding protein 12 ELISA Kit, FK506 binding protein 1Aa ELISA Kit, FK506 binding protein 1C ELISA Kit, FK506-binding protein 59 ELISA Kit, FK506 binding protein like ELISA Kit, FK506 binding protein 1A L homeolog ELISA Kit, FK506 binding protein 1Ab ELISA Kit, Fkbp1a ELISA Kit, FKBP1A ELISA Kit, Fkbp12 ELISA Kit, FKBP12 ELISA Kit, fkbp1aa ELISA Kit, FKBP1C ELISA Kit, EHI_180160 ELISA Kit, LOC5573443 ELISA Kit, Fkbp13 ELISA Kit, FKBPL ELISA Kit, fkbp1a.L ELISA Kit, fkbp1ab ELISA Kit

Gene ID: 2280

UniProt: P62942

Pathways: Negative Regulation of Transporter Activity, Methionine Biosynthetic Process