Casein Kinase 1 gamma 2 ELISA Kit

Catalog No. ABIN6951105

Product Details Casein Kinase 1 gamma 2 ELISA Kit

Target: Casein Kinase 1 gamma 2 (CSNK1G2) (Casein Kinase 1, gamma 2 (CSNK1G2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human CSNK1G2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for Casein Kinase 1 gamma 2

Target: Casein Kinase 1 gamma 2 (CSNK1G2) (Casein Kinase 1, gamma 2 (CSNK1G2))

Alternative Name: CSNK1G2 (CSNK1G2 Products)

Synonyms: CK1g2 ELISA Kit, 2810429I12Rik ELISA Kit, AI463719 ELISA Kit, CKI ELISA Kit, ck1-gamma ELISA Kit, CSNK1G2 ELISA Kit, ck1g2 ELISA Kit, csnk1g2 ELISA Kit, fj97g11 ELISA Kit, wu:fj97g11 ELISA Kit, wz8345 ELISA Kit, zgc:110719 ELISA Kit, zgc:153415 ELISA Kit, casein kinase 1 gamma 2 ELISA Kit, casein kinase 1, gamma 2 ELISA Kit, casein kinase 1 gamma 2 L homeolog ELISA Kit, casein kinase 1, gamma 2a ELISA Kit, casein kinase 1, gamma 2b ELISA Kit, CSNK1G2 ELISA Kit, Csnk1g2 ELISA Kit, csnk1g2.L ELISA Kit, csnk1g2 ELISA Kit, csnk1g2a ELISA Kit, csnk1g2b ELISA Kit

Gene ID: 1455

UniProt: P78368

Pathways: Hedgehog Signaling