BMPR1A ELISA Kit

Catalog No. ABIN6951076

Product Details BMPR1A ELISA Kit

Target: BMPR1A (Bone Morphogenetic Protein Receptor 1A (BMPR1A))

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Bovine BMPR-1A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for BMPR1A

Target: BMPR1A (Bone Morphogenetic Protein Receptor 1A (BMPR1A))

Alternative Name: BMPR-1A (BMPR1A Products)

Synonyms: 10q23del ELISA Kit, ACVRLK3 ELISA Kit, ALK3 ELISA Kit, CD292 ELISA Kit, SKR5 ELISA Kit, 1110037I22Rik ELISA Kit, AU045487 ELISA Kit, BMPR-IA ELISA Kit, Bmpr ELISA Kit, BMPR-1 ELISA Kit, BRK-1 ELISA Kit, BMPR1A ELISA Kit, acvrlk3 ELISA Kit, alk-3 ELISA Kit, alk3 ELISA Kit, cd292 ELISA Kit, skr5 ELISA Kit, BR1a ELISA Kit, alk3tr ELISA Kit, bmpr1a ELISA Kit, zgc:136731 ELISA Kit, wu:fe05b06 ELISA Kit, wu:fe06e10 ELISA Kit, zgc:92227 ELISA Kit, bone morphogenetic protein receptor type 1A ELISA Kit, bone morphogenetic protein receptor, type 1A ELISA Kit, bone morphogenetic protein receptor type 1A S homeolog ELISA Kit, bone morphogenetic protein receptor, type IAa ELISA Kit, bone morphogenetic protein receptor, type IAb ELISA Kit, BMPR1A ELISA Kit, Bmpr1a ELISA Kit, bmpr1a ELISA Kit, bmpr1a.S ELISA Kit, bmpr1aa ELISA Kit, bmpr1ab ELISA Kit

Gene ID: 404101

UniProt: F1MCE7

Pathways: Stem Cell Maintenance