PLAP ELISA Kit

Catalog No. ABIN6951070

Product Details PLAP ELISA Kit

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ALP ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: ALP (ALPP Products)

Synonyms: ECK0378 ELISA Kit, JW0374 ELISA Kit, psiA ELISA Kit, ALP ELISA Kit, PALP ELISA Kit, PLAP ELISA Kit, PLAP-1 ELISA Kit, ILC ELISA Kit, CTACK ELISA Kit, ESkine ELISA Kit, Akp2 ELISA Kit, alp ELISA Kit, zgc:56672 ELISA Kit, DDBDRAFT_0205491 ELISA Kit, DDBDRAFT_0231570 ELISA Kit, DDB_0205491 ELISA Kit, DDB_0231570 ELISA Kit, ALPI ELISA Kit, Actn2lp ELISA Kit, Alp ELISA Kit, bacterial alkaline phosphatase ELISA Kit, alkaline phosphatase, placental ELISA Kit, alkaline phosphatase, placental type ELISA Kit, C-C motif chemokine ligand 27 ELISA Kit, alkaline phosphatase, liver/bone/kidney ELISA Kit, alkaline phosphatase ELISA Kit, alkaline phosphatase, intestinal ELISA Kit, PDZ and LIM domain 3 ELISA Kit, phoA ELISA Kit, ALPP ELISA Kit, LOC100436725 ELISA Kit, CCL27 ELISA Kit, alpl ELISA Kit, alp ELISA Kit, ALPI ELISA Kit, Pdlim3 ELISA Kit, Alpp ELISA Kit

Gene ID: 250

UniProt: P05187