Intestinal Alkaline Phosphatase ELISA Kit

Catalog No. ABIN6951069

Product Details Intestinal Alkaline Phosphatase ELISA Kit

Target: Intestinal Alkaline Phosphatase (ALPI)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ALPI ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Alternative Name: ALPI (ALPI Products)

Synonyms: IAP ELISA Kit, Alp1 ELISA Kit, S51097 ELISA Kit, 2010001C14Rik ELISA Kit, Akp6 ELISA Kit, gIAP ELISA Kit, alpi ELISA Kit, im:7149588 ELISA Kit, si:ch211-262h13.1 ELISA Kit, wu:fa95f04 ELISA Kit, zgc:110794 ELISA Kit, ALPI ELISA Kit, Alpi ELISA Kit, alkaline phosphatase, intestinal ELISA Kit, alkaline phosphatase, intestinal, tandem duplicate 1 ELISA Kit, intestinal-type alkaline phosphatase ELISA Kit, ALPI ELISA Kit, Alpi ELISA Kit, alpi.1 ELISA Kit, LOC100724954 ELISA Kit

Gene ID: 248

UniProt: P09923