Adenylate Kinase 1 ELISA Kit
-
- Target See all Adenylate Kinase 1 (AK1) ELISA Kits
- Adenylate Kinase 1 (AK1)
-
Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Rat AK1 ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Characteristics
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product AK1 ELISA Kit
-
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
-
- Expiry Date
- 6 months
-
- Target See all Adenylate Kinase 1 (AK1) ELISA Kits
- Adenylate Kinase 1 (AK1)
- Alternative Name
- AK1 (AK1 Products)
- Synonyms
- ADK-1 ELISA Kit, AK1 ELISA Kit, CG17146 ELISA Kit, DAK1 ELISA Kit, Dak1 ELISA Kit, Dmel\\CG17146 ELISA Kit, adk1 ELISA Kit, bs34e10.y1 ELISA Kit, ak5 ELISA Kit, ADENYLATE KINASE 1 ELISA Kit, MLE2.3 ELISA Kit, MLE2_3 ELISA Kit, adenylate kinase 1 ELISA Kit, Ak-1 ELISA Kit, B430205N08Rik ELISA Kit, Ak 1 ELISA Kit, zgc:91930 ELISA Kit, Myokinase ELISA Kit, Adenylate kinase 1 ELISA Kit, adenylate kinase 1 ELISA Kit, Adk1 ELISA Kit, ak1 ELISA Kit, AK1 ELISA Kit, ADK1 ELISA Kit, Ak1 ELISA Kit
- Gene ID
- 24183
- UniProt
- P39069
- Pathways
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
-