SETD8 ELISA Kit

Catalog No. ABIN6951049

Product Details SETD8 ELISA Kit

Target: SETD8 (SET Domain Containing (Lysine Methyltransferase) 8 (SETD8))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human SETD8/KMT5A/PRSET7 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for SETD8

Target: SETD8 (SET Domain Containing (Lysine Methyltransferase) 8 (SETD8))

Alternative Name: SETD8 (KMT5A/PRSET7) (SETD8 Products)

Synonyms: KMT5A ELISA Kit, PR-Set7 ELISA Kit, SET07 ELISA Kit, SET8 ELISA Kit, 2410195B05Rik ELISA Kit, AA617402 ELISA Kit, AW536475 ELISA Kit, PR-SET7 ELISA Kit, setd8b ELISA Kit, wu:fc23f12 ELISA Kit, zgc:171649 ELISA Kit, Pr-set7 ELISA Kit, RGD1305893 ELISA Kit, Setd8 ELISA Kit, kmt5a ELISA Kit, kmt5a-a ELISA Kit, setd8 ELISA Kit, setd8-a ELISA Kit, set8a ELISA Kit, setd8a ELISA Kit, wu:fj33f02 ELISA Kit, zgc:153719 ELISA Kit, lysine methyltransferase 5A ELISA Kit, lysine methyltransferase 5Ab ELISA Kit, lysine methyltransferase 5A S homeolog ELISA Kit, lysine methyltransferase 5Aa ELISA Kit, KMT5A ELISA Kit, Kmt5a ELISA Kit, kmt5ab ELISA Kit, kmt5a.S ELISA Kit, kmt5aa ELISA Kit

Gene ID: 387893

UniProt: Q9NQR1