CADM1 ELISA Kit

Catalog No. ABIN6810493

Product Details CADM1 ELISA Kit

Target: CADM1 (Cell Adhesion Molecule 1 (CADM1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse IGSF4A/SynCAM1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse IGSF4A.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for CADM1

Target: CADM1 (Cell Adhesion Molecule 1 (CADM1))

Alternative Name: IGSF4A (CADM1 Products)

Synonyms: BL2 ELISA Kit, IGSF4 ELISA Kit, IGSF4A ELISA Kit, NECL2 ELISA Kit, Necl-2 ELISA Kit, RA175 ELISA Kit, ST17 ELISA Kit, SYNCAM ELISA Kit, TSLC1 ELISA Kit, sTSLC-1 ELISA Kit, sgIGSF ELISA Kit, synCAM1 ELISA Kit, bl2 ELISA Kit, igsf4 ELISA Kit, igsf4a ELISA Kit, necl-2 ELISA Kit, necl2 ELISA Kit, ra175 ELISA Kit, sgigsf ELISA Kit, st17 ELISA Kit, stslc-1 ELISA Kit, syncam ELISA Kit, syncam1 ELISA Kit, tslc1 ELISA Kit, xnecl2 ELISA Kit, 2900073G06Rik ELISA Kit, 3100001I08Rik ELISA Kit, AI987920 ELISA Kit, Bl2 ELISA Kit, Igsf4 ELISA Kit, Igsf4a ELISA Kit, Necl2 ELISA Kit, RA175A ELISA Kit, RA175B ELISA Kit, RA175C ELISA Kit, RA175N ELISA Kit, SgIGSF ELISA Kit, SynCam ELISA Kit, Tslc1 ELISA Kit, SynCAM1 ELISA Kit, cell adhesion molecule 1 ELISA Kit, cell adhesion molecule 1 L homeolog ELISA Kit, CADM1 ELISA Kit, cadm1.L ELISA Kit, Cadm1 ELISA Kit

UniProt: Q8R5M8

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Activated T Cell Proliferation